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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse IL-1 beta Rabbit Recombinant Antibody

IL-1 beta Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

241364B3

Cat no : 98179-1-RR

Synonyms

IL 1beta, Il1b, IL-1,IL1, IL-1 beta,IL 1 beta,IL1beta, Il 1b

Validation Data Gallery

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  • Flow cytometry (FC) experiment of mouse peritoneal macrophages using Anti-Mouse IL-1 beta Rabbit Recombinant Antibody (98179-1-RR)

    FC experiment of mouse peritoneal macrophages using 98179-1-RR

    1x10^6 untreated or LPS and Brefeldin A treated mouse peritoneal macrophages were intracellularly stained with 0.25 ug Anti-Mouse IL-1 beta Rabbit Recombinant Antibody (98179-1-RR, Clone: 241364B3) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC anti-Mouse CD11b. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98179-1-RR

    Affinity and Kinetic Characterization of 98179-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98179-1-RR against Mouse IL-1 beta/IL-1F2 were performed. The affinity constant is 32.5 pM.

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Tested Applications

Positive FC (Intra) detected inLPS and Brefeldin A treated mouse peritoneal macrophages

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98179-1-RR targets IL-1 beta in FC (Intra) applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 1 beta
Calculated molecular weight31kDa
GenBank accession numberNM_008361.4
Gene symbol IL-1 beta
Gene ID (NCBI) 16176
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor Il-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions (PMID: 8630372; PMID: 12401481; PMID: 23704929; PMID: 24618930).

Protocols

Product Specific Protocols
FC protocol for IL-1 beta antibody 98179-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols