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  • Featured Product
  • KD/KO Validated

IL-10 Monoclonal antibody, PBS Only

IL-10 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8E4B6

Cat no : 60269-1-PBS

Synonyms

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-10 Monoclonal antibody (60269-1-Ig)

    WB analysis of Recombinant protein using 60269-1-Ig (same clone as 60269-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60269-1-Ig (IL-10 antibody at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IL-10 Monoclonal antibody (60269-1-Ig)

    IHC staining of human pancreas cancer using 60269-1-Ig (same clone as 60269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60269-1-Ig (IL-10 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IL-10 Monoclonal antibody (60269-1-Ig)

    IHC staining of human pancreas cancer using 60269-1-Ig (same clone as 60269-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60269-1-Ig (IL-10 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MOLT-4 cells using IL-10 Monoclonal antibody (60269-1-Ig)

    IF Staining of MOLT-4 using 60269-1-Ig (same clone as 60269-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MOLT-4 cells using IL-10 antibody (60269-1-Ig, Clone: 8E4B6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60269-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60269-1-PBS targets IL-10 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-10 fusion protein Ag14870 相同性解析による交差性が予測される生物種
Full Name interleukin 10
Calculated molecular weight 21 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC104252
Gene symbol IL10
Gene ID (NCBI) 3586
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin (IL)-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.