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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-10 Recombinant antibody

IL-10 Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240430G12

Cat no : 82191-3-RR

Synonyms

CSIF, Il 10, Il10, interleukin 10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-10 Recombinant antibody (82191-3-RR)

    WB analysis using 82191-3-RR

    Untreated, PMA, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 82191-3-RR (Il10 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of RAW 264.7 cells using IL-10 Recombinant antibody (82191-3-RR)

    IF Staining of RAW 264.7 using 82191-3-RR

    Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using Il10 antibody (82191-3-RR, Clone: 240430G12 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inRAW 264.7 cells
Positive IF detected inLPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82191-3-RR is IL-10 Fusion Protein expressed in E. coli.

Tested Reactivity Mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 10
Calculated molecular weight 21 kDa
Observed molecular weight20 kDa
GenBank accession numberNM_010548
Gene symbol Il10
Gene ID (NCBI) 16153
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-10 is an anti-inflammatory cytokine, produced by T helper (Th) cells, macrophages, monocytes, and B cells, that plays a crucial role in preventing inflammatory and autoimmune pathologies. It downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. IL-10 can block NF-κB activity, and is involved in the regulation of the JAK-STAT signaling pathway. IL-10, along with its receptors, describes an important role in pathogenesis of various diseases, including infectious, inflammatory, autoimmune diseases. IL-10 mutations are associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis.

Protocols

Product Specific Protocols
WB protocol for IL-10 antibody 82191-3-RRDownload protocol
IF protocol for IL-10 antibody 82191-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols