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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-11 Monoclonal antibody, PBS Only (Detector)

IL-11 Monoclonal Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

1F3A12

Cat no : 68135-1-PBS

Synonyms

IL11, interleukin 11, IL 11, AGIF, Adipogenesis inhibitory factor

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-11 Monoclonal antibody (68135-1-Ig)

    WB analysis using 68135-1-Ig (same clone as 68135-1-PBS)

    Saos-2 cell supernatant (cells stimulated with 500ng/mL HGF for 4 days) were subjected to SDS PAGE followed by western blot with 68135-1-Ig (IL-11 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68135-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50054-1

    Sandwich ELISA standard curve of MP50054-1

    Sandwich ELISA standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair - PBS only. 68135-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Ag11199. 68135-1-PBS was biotinylated as the detection antibody. Range: 6.25-400 pg/mL.

  • Sample test of MP50054-1

    Sample test of MP50054-1

    Cytometric bead array of Saos-2 cells supernatant using MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Saos-2 cells were inoculated into 6 well plate at 40000 cells/well followed by culture for 3 days in normal culture media or HGF (Cat.NO. HZ-1084, 500 ng/mL) containing media. 50 μL of the supernatant from each day was analyzed using MP50054-1 (IL11 Monoclonal Matched Antibody Pair). The signal (Median Fluorescenence Intensity) is highly correlated with the stimulation time. Non-related target B2M Monoclonal Matched Antibody Pair (MP50108-1) was served as control.

  • Cytometric bead array standard curve of MP50054-1

    Cytometric bead array standard curve of MP50054-1

    Cytometric bead array standard curve of MP50054-1, IL11 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68135-2-PBS. Detection antibody: 68135-1-PBS. Standard:Ag11199. Range: 1.563-100 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68135-1-PBS targets IL-11 as part of a matched antibody pair:

MP50054-1: 68135-2-PBS capture and 68135-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Affinity
KD=1.80 x 10-9M
KOff=2.20 x 10-6M
KOn=1.22 x 103M
Immunogen IL-11 fusion protein Ag11199 相同性解析による交差性が予測される生物種
Full Name interleukin 11
Calculated molecular weight 199 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC012506
Gene symbol IL-11
Gene ID (NCBI) 3589
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-11 (IL-11) is a member of the glycoprotein-130 (GP-130) cytokines that utilizes the GP-130 signaling pathway shared by other cytokines of the same family. The most well-known role of IL-11 is in hematopoiesis because it promotes megakaryocytopoiesis, erythropoiesis, and thrombopoiesis. In recognition of these properties, human IL-11 recombinant protein (rhIL-11) is used clinically for treating chemotherapy-induced thrombocytopenia. IL-11 has extensive immunomodulatory effects, both anti- and proinflammatory.