Validation Data Gallery
Tested Applications
| Positive FC (Intra) detected in | IFNγ and LPS treated RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
98004-1-PBS targets IL-12/IL-23 p40 in FC (Intra) applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
| Full Name | IL-12/IL-23 p40 |
| Calculated molecular weight | 70 kDa |
| Gene Symbol | |
| Gene ID (NCBI) | |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P43432 |
| Storage Buffer | PBS only , pH 7.3 |
| Storage Conditions | Store at -80°C. Stable for one year after shipment. |
Background Information
IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IL-12/IL-23 p40 antibody 98004-1-PBS | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |