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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-12 p70 Recombinant antibody (82878-2-RR)

    WB analysis using 82878-2-RR

    Recombinant protein Eg0742 (IL-12 p70) and Recombinant protein Eg1821 (IL-12A p35)were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Recombinant protein using IL-12 p70 Recombinant antibody (82878-2-RR)

    WB analysis of Recombinant protein using 82878-2-RR

    Recombinant protein Eg0742 (IL-12 p70) were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82878-2-RR

    Affinity and Kinetic Characterization of 82878-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82878-2-RR against Mouse IL-12 p70 were performed. The affinity constant is 7.37 nM.

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IL-12 p70 Recombinant antibody

IL-12 p70 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 82878-2-RR
Clone No.230037B7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

Il 12b, Il 12p40, IL-12 P35, IL-12 p70, Il12b, Il12p40, interleukin 12b, p40

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82878-2-RR targets IL-12 p70 in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name IL-12 p70
Calculated molecular weight70 kDa
Observed molecular weight70 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_3670597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP43432(IL12B);P43431(IL12A)
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.

Protocols

Product Specific Protocols
WB protocol for IL-12 p70 antibody 82878-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols