• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-12 p70 Recombinant antibody

IL-12 p70 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230037B7

Cat no : 82878-2-RR

Synonyms

Il 12b, Il 12p40, IL-12 P35, IL-12 p70, Il12b, Il12p40, interleukin 12b, p40

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Recombinant protein using IL-12 p70 Recombinant antibody (82878-2-RR)

    WB analysis of Recombinant protein using 82878-2-RR

    Recombinant protein Eg0742 (IL-12 p70) were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IL-12 p70 Recombinant antibody (82878-2-RR)

    WB analysis using 82878-2-RR

    Recombinant protein Eg0742 (IL-12 p70) and Recombinant protein Eg1821 (IL-12A p35)were subjected to SDS PAGE followed by western blot with 82878-2-RR (IL-12 p70 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 82878-2-RR

    Affinity and Kinetic Characterization of 82878-2-RR

    Biolayer interferometry (BLl) kinetic assays of 82878-2-RR against Mouse IL-12 p70 were performed. The affinity constant is 7.37 nM.

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82878-2-RR is IL-12 p70 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name IL-12 p70
Calculated molecular weight70 kDa
Observed molecular weight70 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_3670597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 is a 75 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40) that is secreted by a wide variety of antigen presenting cells (APCs), including phagocytes, B cells and Langerhans cells.

Protocols

Product Specific Protocols
WB protocol for IL-12 p70 antibody 82878-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols