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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-12/IL-23 p40 Polyclonal antibody

IL-12/IL-23 p40 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 30956-1-AP

Synonyms

IL12B, IL 23, IL 12B, IL 12 subunit p40, IL 12 P40

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-12B Polyclonal antibody (30956-1-AP)

    WB analysis using 30956-1-AP

    Human IL-12B/p40, IL-12/p70, IL-12A/p35 recombinant protein, were subjected to SDS PAGE followed by western blot with 30956-1-AP (IL-12B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP)

    WB analysis using 30956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30956-1-AP (IL-12B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of Jurkat cells using IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP)

    FC experiment of Jurkat using 30956-1-AP

    1x10^6 Jurkat cells were intracellularly stained with 0.4 ug IL-12/IL-23 p40 Polyclonal antibody (30956-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inRecombinant protein, HepG2 cells, Jurkat cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30956-1-AP targets IL-12/IL-23 p40 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)
Calculated molecular weight 328 aa, 37 kDa
Observed molecular weight40-45 kDa
GenBank accession numberBC067498
Gene symbol IL12B
Gene ID (NCBI) 3593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-12 and IL-23 are heterodimeric cytokines that share a common p40 subunit (PMID: 11114383). IL-12 is composed of the IL-12 p40 subunit linked to the IL-12 p35 subunit, and the heterodimer signals through the IL-12 receptor (IL-12R), which comprises the IL-12Rβ1 and IL-12Rβ2 subunits. IL-23 is composed of the IL-23 p19 subunit and the IL-12 p40 (IL-12/23p40) subunit, which signals through IL-23R and IL-12Rβ1 (PMID: 11114383; 26121196 ). IL-12/IL-23 p40 also exists as a monomer and as a homodimer which can act as a potent IL-12 antagonist (PMID: 8958912; 18783467). IL-12/IL-23 p40 is produced by antigen-presenting cells, such as dendritic cells (DCs), monocytes, macrophages, neutrophils and, to a lesser extent, B cells (PMID: 20476918).

Protocols

Product Specific Protocols
WB protocol for IL-12/IL-23 p40 antibody 30956-1-APDownload protocol
FC protocol for IL-12/IL-23 p40 antibody 30956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols