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  • Featured Product
  • KD/KO Validated

IL-12RB1 Polyclonal antibody, PBS Only

IL-12RB1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13287-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using IL-12RB1 Polyclonal antibody (13287-1-AP)

    WB analysis of K-562 using 13287-1-AP (same clone as 13287-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 13287-1-AP (IL12RB1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13287-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IL-12RB1 Polyclonal antibody (13287-1-AP)

    WB analysis of Jurkat using 13287-1-AP (same clone as 13287-1-PBS)

    WB result of IL-12RB1 antibody (13287-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12RB1 transfected Jurkat cells. This data was developed using the same antibody clone with 13287-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of K-562 cells using IL-12RB1 Polyclonal antibody (13287-1-AP)

    IP experiment of K-562 using 13287-1-AP (same clone as 13287-1-PBS)

    IP result of anti-IL-12RB1 (IP:13287-1-AP, 4ug; Detection:13287-1-AP 1:300) with K-562 cells lysate 1820 ug. This data was developed using the same antibody clone with 13287-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-12RB1 Polyclonal antibody (13287-1-AP)

    IHC staining of human tonsillitis using 13287-1-AP (same clone as 13287-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 13287-1-AP (IL12RB1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13287-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

13287-1-PBS targets IL-12RB1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-12RB1 fusion protein Ag3983 相同性解析による交差性が予測される生物種
Full Name interleukin 12 receptor, beta 1
Calculated molecular weight 662 aa, 73 kDa
Observed molecular weight 100 kDa
GenBank accession numberBC029121
Gene symbol IL12RB1
Gene ID (NCBI) 3594
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.