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Validation Data Gallery

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  • Western Blot (WB) analysis of Ramos cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of Ramos using 66829-1-Ig (same clone as 66829-1-PBS)

    Ramos cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66829-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis using 66829-1-Ig (same clone as 66829-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66829-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of Jurkat using 66829-1-Ig (same clone as 66829-1-PBS)

    WB result of IL-12RB1 antibody (66829-1-Ig; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-IL-12RB1 transfected Jurkat cells. This data was developed using the same antibody clone with 66829-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of Raji using 66829-1-Ig (same clone as 66829-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66829-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of THP-1 using 66829-1-Ig (same clone as 66829-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66829-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    WB analysis of Jurkat using 66829-1-Ig (same clone as 66829-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66829-1-Ig (IL12RB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66829-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using IL-12RB1 Monoclonal antibody (66829-1-Ig)

    IF Staining of THP-1 using 66829-1-Ig (same clone as 66829-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL-12RB1 antibody (66829-1-Ig, Clone: 1E10E10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66829-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

IL-12RB1 Monoclonal antibody, PBS Only

IL-12RB1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66829-1-PBS
Clone No.1E10E10

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

IL12RB1, IL-12 receptor beta component, IL 12RB1, IL 12R subunit beta 1, IL 12R beta 1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66829-1-PBS targets IL-12RB1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-12RB1 fusion protein Ag6920 相同性解析による交差性が予測される生物種
Full Name interleukin 12 receptor, beta 1
Calculated molecular weight 662 aa, 73 kDa
Observed molecular weight 100-110 kDa
GenBank accession numberBC029121
Gene Symbol IL12RB1
Gene ID (NCBI) 3594
RRIDAB_2882172
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP42701
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL12RB1, also named as Interleukin-12 receptor subunit beta-1 or CD212, is a 662 amino acid protein, which contains 5 fibronectin type-III domains and belongs to the type I cytokine receptor family. Type 2 subfamily. IL12RB1 localizes in the membrane and functions as an interleukin receptor which binds interleukin-12 with low affinity and is involved in IL12 transduction. Associated with IL12RB2 it forms a functional, high affinity receptor for IL12. Associates also with IL23R to form the interleukin-23 receptor which functions in IL23 signal transduction probably through activation of the Jak-Stat signaling cascade.