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Species Reactivity
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-13 Recombinant Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Rabbit / IgG

Detection: Rabbit / IgG

Reactivity

Capture: human

Detection: human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 62.5-4000 pg/mL

Cat No : MP01078-3

Print datasheet

Synonyms

Validation Data Gallery

  • Sandwich ELISA standard curve of MP01078-3, Human IL-13 Recombinant Matched Antibody Pair - PBS only. 84158-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0061. 84158-3-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

    Sandwich ELISA standard curve of MP01078-3

    Sandwich ELISA standard curve of MP01078-3, Human IL-13 Recombinant Matched Antibody Pair - PBS only. 84158-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0061. 84158-3-PBS was HRP conjugated as the detection antibody. Range: 62.5-4000 pg/mL

  • Serum of eight individual healthy human donors was measured. The human IL-13 concentration of detected samples was determined to be 50.3 pg/mL with a range of 31.9 - 429.1 pg/mL.

    Sample test of MP01078-3

    Serum of eight individual healthy human donors was measured. The human IL-13 concentration of detected samples was determined to be 50.3 pg/mL with a range of 31.9 - 429.1 pg/mL.

  • Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean IL-13 concentration was determined to be 101.1 pg/mL in unstimulated PBMC supernatant, 2,738.4 pg/mL in PHA stimulated PBMC supernatant.

    Sample test of MP01078-3

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean IL-13 concentration was determined to be 101.1 pg/mL in unstimulated PBMC supernatant, 2,738.4 pg/mL in PHA stimulated PBMC supernatant.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP01078-3 targets IL-13 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 62.5-4000 pg/mL (Sandwich ELISA);

Capture antibody: IL13 Recombinant antibody, PBS Only (Capture) 84158-5-PBS (241396H6). 100 μg. Concentration 1 mgl/ml.

Detection antibody: IL-13 Recombinant antibody, PBS Only (Detector) 84158-3-PBS (241396E12). 100 μg. Concentration 1 mgl/ml.

Alternative IL-13 matched antibody pairs: MP01078-1, MP01078-2

Matched pair protein standard: Eg0061

Unconjugated rabbit recombinant monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 84158-5-PBS
Tested Reactivity human
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 13
Synonyms IL-13, NC30, Interleukin-13, interleukin 13, IL 13
GenBank accession numberAAK53823
Gene symbol IL-13
UniprotP35225
Gene ID (NCBI) 3596
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 84158-3-PBS
Tested Reactivity human
Immunogen Recombinant protein
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name interleukin 13
Synonyms IL13, NC30, Interleukin-13, interleukin 13, IL 13
GenBank accession numberAAK53823
Gene symbol IL-13
UniprotP35225
Gene ID (NCBI) 3596
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.