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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-13Ra1 Recombinant antibody

IL-13Ra1 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241834G10

Cat no : 84525-4-RR

Synonyms

IL13RA1, IL 13R alpha 1, IL 13 receptor subunit alpha 1, CT19, CD213A1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-13Ra1 Recombinant antibody (84525-4-RR)

    WB analysis using 84525-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84525-4-RR (IL-13Ra1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHUVEC cells, HT-29 cells, K-562 cells, A549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84525-4-RR targets IL-13Ra1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FusionProtein 相同性解析による交差性が予測される生物種
Full Name interleukin 13 receptor, alpha 1
Calculated molecular weight 49 kDa
Observed molecular weight70-75 kDa
GenBank accession numberBC009960
Gene symbol IL-13Rα1
Gene ID (NCBI) 3597
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-13 is an immunoregulatory cytokine secreted predominantly by activated T-helper 2 (Th2) cells and performs multiple functions through a complex receptor system comprised of IL-4Rα and two IL-13 binding proteins, IL-13Rα1 and IL-13Rα2 (PMID: 12704343). IL-13Rα1 is a glycosylated transmembrane protein (PMID: 10856136; 9723655). IL-13Rα1 is expressed on a wide range of cells including B cells, monocytes, macrophages, basophils, mast cells, and endothelial cells (PMID: 10856136; 31866859). IL-13 binding to the IL-4Rα/IL-13Rα1 complex leads to activation of JAK1, JAK2, and TYK2 and subsequent phosphorylation of STAT6 and STAT3 (PMID: 31866859).

Protocols

Product Specific Protocols
WB protocol for IL-13Ra1 antibody 84525-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols