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  • Featured Product
  • KD/KO Validated

IL-17A Monoclonal antibody, PBS Only

IL-17A Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1B3D5

Cat no : 66148-1-PBS

Synonyms

IL 17, IL17, IL-17, IL17A, IL 17A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-17A Monoclonal antibody (66148-1-Ig)

    WB analysis using 66148-1-Ig (same clone as 66148-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using IL-17A Monoclonal antibody (66148-1-Ig)

    WB analysis of Jurkat using 66148-1-Ig (same clone as 66148-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of U-937 cells using IL-17A Monoclonal antibody (66148-1-Ig)

    WB analysis of U-937 using 66148-1-Ig (same clone as 66148-1-PBS)

    U-937 cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Raji cells using IL-17A Monoclonal antibody (66148-1-Ig)

    WB analysis of Raji using 66148-1-Ig (same clone as 66148-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66148-1-Ig (IL-17 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IL-17A Monoclonal antibody (66148-1-Ig)

    IHC staining of human skin cancer using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IL-17A Monoclonal antibody (66148-1-Ig)

    IHC staining of human skin cancer using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66148-1-Ig (IL-17A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using IL-17A Monoclonal antibody (66148-1-Ig)

    IHC staining of human colon using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using IL-17A Monoclonal antibody (66148-1-Ig)

    IHC staining of human colon using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IL-17A Monoclonal antibody (66148-1-Ig)

    IHC staining of human colon cancer using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IL-17A Monoclonal antibody (66148-1-Ig)

    IHC staining of human colon cancer using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66148-1-Ig (IL-17 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-17A Monoclonal antibody (66148-1-Ig)

    IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-17A Monoclonal antibody (66148-1-Ig)

    IF Staining of human tonsillitis using 66148-1-Ig (same clone as 66148-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-17A antibody (66148-1-Ig, Clone: 1B3D5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66148-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66148-1-PBS targets IL-17A in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-17A fusion protein Ag3733 相同性解析による交差性が予測される生物種
Full Name interleukin 17A
Calculated molecular weight 155 aa, 18 kDa
Observed molecular weight 18 kDa
GenBank accession numberBC067505
Gene symbol IL-17A
Gene ID (NCBI) 3605
RRIDAB_2881544
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.