IL-17A Recombinant antibody
IL-17A Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
230038B1
Cat no : 82905-1-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PMA, Ionomycin and Brefeldin A treated EL-4 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:1000-1:5000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
82905-1-RR targets IL-17A in WB, ELISA applications and shows reactivity with mouse samples.
Tested Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Recombinant protein 相同性解析による交差性が予測される生物種 |
Full Name | interleukin 17A |
Calculated molecular weight | 17 kDa |
Observed molecular weight | 14 kDa, 17 kDa |
GenBank accession number | NM-010552 |
Gene symbol | Il17a |
Gene ID (NCBI) | 16171 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL17A, also named as IL-17, is a proinflammatory cytokine. IL-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The IL-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. IL-17 and IL-22, are highly increased.
Protocols
Product Specific Protocols | |
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WB protocol for IL-17A antibody 82905-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |