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  • Featured Product
  • KD/KO Validated

IL-18 Monoclonal antibody, PBS Only

IL-18 Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5C6F8

Cat no : 60070-1-PBS

Synonyms

IL18, Pre-IL18, IL-1 gamma, IL 18, IL 1 gamma

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-18 Monoclonal antibody (60070-1-Ig)

    WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL18 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using IL-18 Monoclonal antibody (60070-1-Ig)

    WB analysis of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

    WB result of Pre-IL18 antibody (60070-1-Ig, 1:4000) with si-Control and si-Pre-IL18 transfected HeLa cells.. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using IL-18 Monoclonal antibody (60070-1-Ig)

    WB analysis using 60070-1-Ig (same clone as 60070-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60070-1-Ig (IL-18 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-18 Monoclonal antibody (60070-1-Ig)

    IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-18 Monoclonal antibody (60070-1-Ig)

    IHC staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60070-1-Ig (IL18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using IL-18 Monoclonal antibody (60070-1-Ig)

    IHC staining of human stomach cancer using 60070-1-Ig (same clone as 60070-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using IL-18 Monoclonal antibody (60070-1-Ig)

    IHC staining of human stomach cancer using 60070-1-Ig (same clone as 60070-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60070-1-Ig (IL-18 antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-18 Monoclonal antibody (60070-1-Ig)

    IF Staining of human tonsillitis using 60070-1-Ig (same clone as 60070-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-18 antibody (60070-1-Ig, Clone: 5C6F8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using IL-18 Monoclonal antibody (60070-1-Ig)

    FC experiment of HeLa using 60070-1-Ig (same clone as 60070-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug IL-18 Monoclonal antibody (60070-1-Ig, Clone:5C6F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60070-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60070-1-PBS targets IL-18 in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-18 fusion protein Ag1063 相同性解析による交差性が予測される生物種
Full Name IL-18
Calculated molecular weight 193 aa, 22 kDa
Observed molecular weight 24 kDa
GenBank accession numberBC007461
Gene symbol IL-18
Gene ID (NCBI) 3606
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 24 kDa precursor and then cleaved into a biologically activate 18 kDa form.