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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-19 Monoclonal antibody, PBS Only

IL-19 Monoclonal Antibody for IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

8H10H8

Cat no : 60278-1-PBS

Synonyms

IL 10C, IL 19, IL19, IL-19, interleukin 19, MDA1, NG.1, ZMDA1

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-19 Monoclonal antibody (60278-1-Ig)

    WB analysis of Recombinant protein using 60278-1-Ig (same clone as 60278-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60278-1-Ig (IL-19 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Recombinant protein using IL-19 Monoclonal antibody (60278-1-Ig)

    WB analysis of Recombinant protein using 60278-1-Ig (same clone as 60278-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60278-1-Ig (IL-19 antibody at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IHC staining of human tonsillitis using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IHC staining of human tonsillitis using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IHC staining of human breast cancer using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IHC staining of human breast cancer using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IHC staining of human skin cancer using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IHC staining of human skin cancer using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IHC staining of human tonsillitis using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IHC staining of human tonsillitis using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60278-1-Ig (IL-19 antibody at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IF Staining of human tonsillitis using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-19 Monoclonal antibody (60278-1-Ig)

    IF Staining of human tonsillitis using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-937 cells using IL-19 Monoclonal antibody (60278-1-Ig)

    IF Staining of U-937 using 60278-1-Ig (same clone as 60278-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells using IL-19 antibody (60278-1-Ig, Clone: 8H10H8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60278-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60278-1-PBS targets IL-19 in IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-19 fusion protein Ag18887 相同性解析による交差性が予測される生物種
Full Name interleukin 19
Calculated molecular weight 177 aa, 20 kDa
GenBank accession numberBC153127
Gene symbol IL19
Gene ID (NCBI) 29949
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

IL-19, a member of the IL-10 cytokine family, is expressed in epithelial cells, endothelial cells, and macrophages. It can bind the interleukin-20 receptor complex IL-20R1/IL-20R2 and lead to the activation of the signal transducer and activator of transcription 3 (STAT3). IL-19 induces IL-6 and TNF-alpha production in monocytes. It also induces cell apoptosis and reactive oxygen species production in monocytes, which suggests a role of this cytokine in inflammatory responses. It is up-regulated in monocytes following stimulation with lipopolysaccharide (LPS), and granulocyte-macrophage colony-stimulating factor (GM-CSF). IL-19 alters the balance of Th1 and Th2 cells in favor of Th2 cells. IL-19 contributes to a range of diseases and disorders, such as breast cancer, squamous cell carcinoma of the skin, asthma, endotoxic shock, uremia, psoriasis, rheumatoid arthritis, and periodontal and vascular disease.