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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-1F10 Monoclonal antibody (60319-1-Ig)

    WB analysis of Recombinant protein using 60319-1-Ig (same clone as 60319-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60319-1-Ig (IL1F10 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60319-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-937 cells using IL-1F10 Monoclonal antibody (60319-1-Ig)

    FC experiment of U-937 using 60319-1-Ig (same clone as 60319-1-PBS)

    1X10^6 U-937 cells were intracellularly stained with 0.4 ug Anti-Human IL-1F10 (60319-1-Ig, Clone:5B2F5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60319-1-PBS in a different storage buffer formulation.

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IL-1F10 Monoclonal antibody, PBS Only

IL-1F10 Monoclonal Antibody for WB, Indirect ELISA
Cat No. 60319-1-PBS
Clone No.5B2F5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

FIL1 theta, FIL1T, FKSG75, IL 1 theta, IL 1F10, IL 1HY2, IL1 theta, IL1F10, IL-1F10, IL1HY2, IL38, Interleukin 1 family member 10, Interleukin 1 HY2, Interleukin 1 theta, UNQ6119/PRO20041

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60319-1-PBS targets IL-1F10 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-1F10 fusion protein Ag17250 相同性解析による交差性が予測される生物種
Full Name interleukin 1 family, member 10 (theta)
Calculated molecular weight 152 aa, 17 kDa
GenBank accession numberBC103966
Gene Symbol IL1F10
Gene ID (NCBI) 84639
RRIDAB_2881430
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ8WWZ1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

IL1F10 is a member of the interleukin 1 cytokine family. This cytokine is thought to participate in a network of interleukin 1 family members to regulate adapted and innate immune responses.