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  • Featured Product
  • KD/KO Validated

IL-1R2 Monoclonal antibody, PBS Only

IL-1R2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3H4H4

Cat no : 60262-1-PBS

Synonyms

IL1R2, IL 1RT 2, IL 1R beta, IL 1R 2, CD121b

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using IL-1R2 Monoclonal antibody (60262-1-Ig)

    WB analysis of HeLa using 60262-1-Ig (same clone as 60262-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 60262-1-Ig (IL1R2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60262-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using IL-1R2 Monoclonal antibody (60262-1-Ig)

    IF Staining of HeLa using 60262-1-Ig (same clone as 60262-1-PBS)

    Immunofluorescent analysis of HeLa cells using 60262-1-Ig (IL1R2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 60262-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60262-1-PBS targets IL-1R2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-1R2 fusion protein Ag4495 相同性解析による交差性が予測される生物種
Full Name interleukin 1 receptor, type II
Calculated molecular weight 398 aa, 45 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC039031
Gene symbol IL1R2
Gene ID (NCBI) 7850
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-1 receptor type 2 (IL1R2, also known as CD121b) is a cytokine receptor that belongs to the interleukin 1 receptor family. It exists in both a membrane bound and soluble form. IL1R2 binds interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA), and acts as a decoy receptor that inhibits the activity of its ligands. IL1R2 serves as a negative regulator of IL-1 signaling, thereby suppressing inflammatory response.