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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-2 Polyclonal antibody

IL-2 Polyclonal Antibody for ELISA, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

ELISA, FC (Intra)

Conjugate

Unconjugated

Cat no : 30199-1-AP

Synonyms

Il2, IL-2, interleukin 2

Validation Data Gallery

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  • Flow cytometry (FC) experiment of rat splenocytes cells using IL-2 Polyclonal antibody (30199-1-AP)

    FC experiment of rat splenocytes using 30199-1-AP

    1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of rat splenocytes cells using IL-2 Polyclonal antibody (30199-1-AP)

    FC experiment of rat splenocytes using 30199-1-AP

    1X10^6 PMA and ionomycin treated rat splenocytes cells were intracellularly stained with 0.5 ug Anti-Rat IL-2 (30199-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, and 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ), and 0.5 ug CoraLite®594 Anti-Rat CD8a (OX-8) (CL594-65180, Clone: OX-8). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inPMA and ionomycin treated rat splenocytes cells
Positive FC detected inPMA and ionomycin treated rat splenocytes cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30199-1-AP targets IL-2 in ELISA, FC (Intra) applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 2
Calculated molecular weight18 kDa
GenBank accession numberNM_053836
Gene symbol Il2
Gene ID (NCBI) 116562
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

Protocols

Product Specific Protocols
FC protocol for IL-2 antibody 30199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols