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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-2 Polyclonal antibody

IL-2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31506-1-AP

Synonyms

Aldesleukin, IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-2 Polyclonal antibody (31506-1-AP)

    WB analysis using 31506-1-AP

    Untreated, PMA, A23187 and Brefeldin A treated were subjected to SDS PAGE followed by western blot with 31506-1-AP (IL-2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inPMA, A23187 and Brefeldin A treated Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31506-1-AP targets IL-2 in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 2
Calculated molecular weight 153 aa, 18 kDa
Observed molecular weight15-18 kDa
GenBank accession numberBC070338
Gene symbol IL2
Gene ID (NCBI) 3558
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL2 is a secreted cytokine involved in the operation and regulation of immune system. IL2 plays a pivotal role in T-cell activation and effector functions, including T cell proliferation, interferon-γproduction and cytotoxicity. IL2 can stimulate the propagation of lymphocytes and induce the cytotoxic T lymphocytes and lymphokine-activated killer cells for tumor cells. IL2 also influences memory T cell homeostasis through the regulation of memory T cell numbers and enhances major histocompatibility complex II (MHC II) expression on tumor cells.

Protocols

Product Specific Protocols
WB protocol for IL-2 antibody 31506-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols