IL-2 Recombinant antibody
IL-2 Recombinant Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
mouse
Applications
WB, ELISA
Conjugate
Unconjugated
CloneNo.
240358B1
Cat no : 83547-3-RR
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | PMA(50ng/ml)+iono(500ng/ml)共6h换液+Bref A 18h EL-4 cells |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
The immunogen of 83547-3-RR is IL-2 Fusion Protein expressed in E. coli.
Tested Reactivity | mouse |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | Fusion Protein 相同性解析による交差性が予測される生物種 |
Full Name | interleukin 2 |
Calculated molecular weight | 19 kDa |
Observed molecular weight | 15 kDa |
GenBank accession number | NM_008366 |
Gene symbol | IL-2 |
Gene ID (NCBI) | 16183 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-2 (Il2) is a protein initially identified as a T cell growth factor. Il2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that Il2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (Il2R). Il2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.
Protocols
Product Specific Protocols | |
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WB protocol for IL-2 antibody 83547-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |