Validation Data Gallery
Tested Applications
| Positive WB detected in | PMA(50ng/ml)+iono(500ng/ml)共6h换液+Bref A 18h EL-4 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83547-3-RR targets IL-2 in WB, ELISA applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen | Fusion Protein 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 2 |
| Calculated molecular weight | 19 kDa |
| Observed molecular weight | 15 kDa |
| GenBank accession number | NM_008366 |
| Gene Symbol | IL-2 |
| Gene ID (NCBI) | 16183 |
| RRID | AB_3671166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04351 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Interleukin-2 (Il2) is a protein initially identified as a T cell growth factor. Il2 is mainly produced by activated CD4+CD25- (helper) T cells, and induces the proliferation of these and others cells like CD8+ T cells, B and NK cells. For this reason, it has been used to treat immune-deficiencies like HIV, and induce immune response against tumors. Nevertheless, it has been shown that Il2 also acts as the main growth factor of CD4+CD25+ Regulatory T cells. Interleukin 2 mediates its functions in the target cell through the interleukin 2 receptor (Il2R). Il2R is a multimeric functional protein consisting of three different chains: Il2Rα, Il2Rβ and γc.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-2 antibody 83547-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |