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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using IL-22RA2 Monoclonal antibody (66190-1-Ig)

    WB analysis of Raji using 66190-1-Ig (same clone as 66190-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL-22RA2 Monoclonal antibody (66190-1-Ig)

    IHC staining of human small intestine using 66190-1-Ig (same clone as 66190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using IL-22RA2 Monoclonal antibody (66190-1-Ig)

    IHC staining of human small intestine using 66190-1-Ig (same clone as 66190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-22RA2 Monoclonal antibody (66190-1-Ig)

    IHC staining of human breast cancer using 66190-1-Ig (same clone as 66190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IL-22RA2 Monoclonal antibody (66190-1-Ig)

    IHC staining of human lung cancer using 66190-1-Ig (same clone as 66190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using IL-22RA2 Monoclonal antibody (66190-1-Ig)

    IHC staining of human lung cancer using 66190-1-Ig (same clone as 66190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using IL-22RA2 Monoclonal antibody (66190-1-Ig)

    IHC staining of human breast cancer using 66190-1-Ig (same clone as 66190-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66190-1-Ig (IL22RA2 Antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using IL-22RA2 Monoclonal antibody (66190-1-Ig)

    IF Staining of human breast cancer using 66190-1-Ig (same clone as 66190-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-22RA2 antibody (66190-1-Ig, Clone: 5G11A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using IL-22RA2 Monoclonal antibody (66190-1-Ig)

    IF Staining of human breast cancer using 66190-1-Ig (same clone as 66190-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using IL-22RA2 antibody (66190-1-Ig, Clone: 5G11A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66190-1-PBS in a different storage buffer formulation.

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IL-22RA2 Monoclonal antibody, PBS Only

IL-22RA2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66190-1-PBS
Clone No.5G11A9

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

CRF2 10, CRF2 S1, CRF2X, IL 22 receptor subunit alpha 2, IL 22BP, IL 22R alpha 2, IL 22RA2, IL22BP, IL22RA2, IL-22RA2, Interleukin 22 binding protein, ZcytoR16

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66190-1-PBS targets IL-22RA2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-22RA2 fusion protein Ag18682 相同性解析による交差性が予測される生物種
Full Name interleukin 22 receptor, alpha 2
Calculated molecular weight 263 aa, 31 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC125167
Gene Symbol IL-22RA2
Gene ID (NCBI) 116379
RRIDAB_2881585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ969J5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Interleukin-22 receptor subunit alpha-2 (IL22RA2) is a soluble member of the type II cytokine receptor family. Through binding to IL22, IL22RA2 prevents the interaction of IL22 with the functional IL22-R complex and blocks the activity of IL22 (in vitro). IL22RA2 may play an important role as an IL22 antagonist in the regulation of inflammatory responses. Alternative splicing results in multiple transcript variants encoding distinct isoforms.