Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
66164-1-PBS targets IL-27A in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IL-27A fusion protein Ag21436 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 27 |
| Calculated molecular weight | 243 aa, 27 kDa |
| Observed molecular weight | 31 kDa |
| GenBank accession number | BC062422 |
| Gene Symbol | IL-27 |
| Gene ID (NCBI) | 246778 |
| RRID | AB_2881560 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q8NEV9 |
| Storage Buffer | PBS Only |
| Storage Conditions | Store at -80°C. |
Background Information
IL-27, as a member of the IL-6/IL-12 family, is a heterodimeric cytokine composed of two subunits: p28 (IL-27a) and EBI3 (IL-27b). IL-27 acts on various cell types, including T cells, B cells, macrophages, dendritic cells, natural killer (NK) cells and non-hematopoietic cells. IL-27 plays a critical role in the early regulation of T helper type 1 initiation, and enhances proliferation of naive CD4+T cells and naive B cells. It, however, also exerts anti-inflammatory functions by inhibiting the development of Th17 cells and inducing IL-10 producing type 1 regulatory T cells. IL-27 is a potentially promising cytokine for therapeutic approaches on various human diseases.