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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-28A Polyclonal antibody

IL-28A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22646-1-AP

Synonyms

Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-28A Polyclonal antibody (22646-1-AP)

    WB analysis of Recombinant protein using 22646-1-AP

    Recombinant protein were subjected to SDS PAGE followed by western blot with 22646-1-AP (IL-28A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-28A Polyclonal antibody (22646-1-AP)

    IHC staining of human tonsillitis using 22646-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22646-1-AP (IL-28A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-28A Polyclonal antibody (22646-1-AP)

    IHC staining of human tonsillitis using 22646-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 22646-1-AP (IL-28A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRecombinant protein protein
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22646-1-AP is IL-28A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-28A fusion protein Ag18525 相同性解析による交差性が予測される生物種
Full Name interleukin 28A (interferon, lambda 2)
Calculated molecular weight 200 aa, 22 kDa
GenBank accession numberBC113583
Gene symbol IL-28A
Gene ID (NCBI) 282616
RRIDAB_2879141
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL28A, also named as IFNL2 and ZCYT020, is a cytokine with immunomodulatory activity. It up-regulates MHC class I antigen expression. IL28A is a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. It plays a significant role in the antiviral immune defense in the intestinal epithelium.

Protocols

Product Specific Protocols
WB protocol for IL-28A antibody 22646-1-APDownload protocol
IHC protocol for IL-28A antibody 22646-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols