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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody, PBS Only

IL-2RG/CD132 Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

242020H6

Cat no : 98234-1-PBS

Synonyms

IL2RG, IL 2RG, IL 2R subunit gamma, IL 2 receptor subunit gamma, gammaC

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibod (98234-1-RR)

    FC experiment of human PBMCs using 98234-1-RR (same clone as 98234-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody (98234-1-RR, Clone: 242020H6), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98234-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human PBMCs using Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibod (98234-1-RR)

    FC experiment of human PBMCs using 98234-1-RR (same clone as 98234-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human IL-2RG/CD132 Rabbit Recombinant Antibody (98234-1-RR, Clone: 242020H6)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98234-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98234-1-RR

    Affinity and Kinetic Characterization of 98234-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98234-1-RR against Human IL-2RG/CD132 were performed. The affinity constant is 1.55 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

98234-1-PBS targets IL-2RG/CD132 in FC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 2 receptor, gamma (severe combined immunodeficiency)
Calculated molecular weightNM_000206.3
GenBank accession numberNM_000206.3
Gene symbol IL2RG
Gene ID (NCBI) 3561
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The interleukin-2 receptor (IL-2R) is a heterotrimeric receptor consisting of three subunits, alpha (IL-2RA), beta (IL-2RB), and gamma (IL-2RG). IL-2RG, also known as CD132, is an important signaling component of many interleukin receptors, including those of interleukin -2, -4, -7 and -21, and is thus referred to as the common gamma chain. Mutations in the gene of IL-2RG cause X-linked severe combined immunodeficiency (XSCID), as well as X-linked combined immunodeficiency (XCID), a less severe immunodeficiency disorder.