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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-33 Monoclonal antibody, PBS Only

IL-33 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2A9C7

Cat no : 66235-1-PBS

Synonyms

C9orf26, DKFZp586H0523, DVS27, IL 1F11, IL 33, IL1F11, IL33, IL-33, Interleukin 1 family member 11, interleukin 33, NF HEV, NFEHEV, NFHEV, RP11 575C20.2

Validation Data Gallery

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  • Western Blot (WB) analysis of human lung tissue using IL-33 Monoclonal antibody (66235-1-Ig)

    WB analysis of human lung using 66235-1-Ig (same clone as 66235-1-PBS)

    human lung tissue were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL33 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of THP-1 cells using IL-33 Monoclonal antibody (66235-1-Ig)

    WB analysis of THP-1 using 66235-1-Ig (same clone as 66235-1-PBS)

    THP-1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of hTERT-RPE1 cells using IL-33 Monoclonal antibody (66235-1-Ig)

    WB analysis of hTERT-RPE1 using 66235-1-Ig (same clone as 66235-1-PBS)

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 66235-1-Ig (IL-33 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IL-33 Monoclonal antibody (66235-1-Ig)

    IHC staining of human pancreas cancer using 66235-1-Ig (same clone as 66235-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using IL-33 Monoclonal antibody (66235-1-Ig)

    IHC staining of human pancreas cancer using 66235-1-Ig (same clone as 66235-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66235-1-Ig (IL-33 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66235-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66235-1-PBS targets IL-33 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen IL-33 fusion protein Ag21430 相同性解析による交差性が予測される生物種
Full Name interleukin 33
Calculated molecular weight 30 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC047085
Gene symbol IL33
Gene ID (NCBI) 90865
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS only
Storage ConditionsStore at -80°C.

Background Information

IL-33 is an IL-1 family cytokine and nuclear alarmin, and is constitutively expressed in epithelial barrier tissues and human blood vessels. IL-33 is involved in the maturation of Th2 cells and the activation of mast cells, basophils, eosinophils and natural killer cells. It also acts as a chemoattractant for Th2 cells, and may function as an "alarmin", that amplifies immune responses during tissue injury.