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Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    WB analysis of Recombinant protein using 60290-1-Ig (same clone as 60290-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60290-1-Ig (IL1F8 Antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    IHC staining of human tonsillitis using 60290-1-Ig (same clone as 60290-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60290-1-Ig (IL36 beta/IL1F8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    IHC staining of human tonsillitis using 60290-1-Ig (same clone as 60290-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60290-1-Ig (IL36 beta/IL1F8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    IF Staining of human tonsillitis using 60290-1-Ig (same clone as 60290-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-36 Beta/IL-1F8 antibody (60290-1-Ig, Clone: 10D12B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-36 Beta/IL-1F8 Monoclonal antibody (60290-1-Ig)

    IF Staining of human tonsillitis using 60290-1-Ig (same clone as 60290-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-36 Beta/IL-1F8 antibody (60290-1-Ig, Clone: 10D12B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60290-1-PBS in a different storage buffer formulation.

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IL-36 Beta/IL-1F8 Monoclonal antibody, PBS Only

IL-36 Beta/IL-1F8 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 60290-1-PBS
Clone No.10D12B1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

FIL1, FIL1 (ETA), FIL1 eta, FIL1H, IL 1 eta, IL 1F8, IL 1H2, IL1 ETA, IL1F8, IL-1F8, IL1H2, IL-36 beta, IL-36 Beta/IL-1F8, IL36B, Interleukin 1 eta, Interleukin 1 family member 8, Interleukin 1 homolog 2, Interleukin-36 beta

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60290-1-PBS targets IL-36 Beta/IL-1F8 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-36 Beta/IL-1F8 fusion protein Ag12675 相同性解析による交差性が予測される生物種
Full Name interleukin 1 family, member 8 (eta)
Calculated molecular weight 157 aa, 18 kDa
Observed molecular weight 44 kDa
GenBank accession numberBC101833
Gene Symbol IL-36 Beta/IL-1F8
Gene ID (NCBI) 27177
RRIDAB_2881406
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9NZH7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.