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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-4 Polyclonal antibody (30533-1-AP)

    IHC staining of human tonsillitis using 30533-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30533-1-AP (IL-4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-4 Polyclonal antibody (30533-1-AP)

    IHC staining of human tonsillitis using 30533-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 30533-1-AP (IL-4 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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IL-4 Polyclonal antibody

IL-4 Polyclonal Antibody for IHC, ELISA
Cat No. 30533-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, ELISA

Synonyms

IL4, Interleukin-4, interleukin 4, IL 4, BSF-1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30533-1-AP targets IL-4 in IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 4
Calculated molecular weight 153 aa, 17 kDa
GenBank accession numberBC070123
Gene Symbol IL-4
Gene ID (NCBI) 3565
RRIDAB_3669731
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05112
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.

Protocols

Product Specific Protocols
IHC protocol for IL-4 antibody 30533-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols