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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-4 Recombinant antibody

IL-4 Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

241249H7

Cat no : 84422-5-RR

Synonyms

Il4, BSF-1, B-cell stimulatory factor 1, B-cell IGG differentiation factor, B-cell growth factor 1

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-4 Recombinant antibody (84422-5-RR)

    WB analysis of Recombinant protein using 84422-5-RR

    Rat IL-4 recombinant protein (Cat No. Eg113) was subjected to SDS PAGE followed by western blot with 84422-5-RR (IL-4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inRecombinant protein

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84422-5-RR targets IL-4 in WB, ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 4
Calculated molecular weight16kDa
GenBank accession numberNM_201270.1
Gene symbol IL-4
Gene ID (NCBI) 287287
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-4 (IL-4) is a pleiotropic cytokine that plays a crucial role in regulating immune responses and mediating type-2 immune reactions. It is primarily produced by T-helper 2 (Th2) cells, mast cells, eosinophils, basophils, and other immune cells. IL-4 exerts its effects by binding to type I and type II receptors, initiating a signaling cascade that involves the phosphorylation of STAT6 and the transcription of IL-4-responsive genes. This pathway is essential for the regulation of immune cell functions and the maintenance of immune homeostasis.

Protocols

Product Specific Protocols
WB protocol for IL-4 antibody 84422-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols