CoraLite® Plus 488-conjugated IL-4 Monoclonal antibody
IL-4 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG2a
Reactivity
human
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1G2C5
Cat no : CL488-66142
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | Ramos cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) | FC : 0.80 ug per 10^6 cells in a 100 µl suspension |
Sample-dependent, check data in validation data gallery |
Product Information
CL488-66142 targets IL-4 in FC (Intra) applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | IL-4 fusion protein Ag9940 相同性解析による交差性が予測される生物種 |
Full Name | interleukin 4 |
Calculated molecular weight | 153 aa, 17 kDa |
Observed molecular weight | 18 kDa |
GenBank accession number | BC070123 |
Gene symbol | IL4 |
Gene ID (NCBI) | 3565 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL4 is a cytokine produced by CD4+ T cells in response to helminthes and other extracellular parasites. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 IL-4 antibody CL488-66142 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |