• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated IL-4R Polyclonal antibody

IL-4R Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28331

Synonyms

CD124, IL 4 binding protein, IL 4 receptor subunit alpha, IL 4R alpha, IL 4R subunit alpha, IL 4RA, IL4 BP, IL4R, IL-4R, IL4RA, interleukin 4 receptor, sIL4Ralpha/prot, Soluble IL 4R alpha

Validation Data Gallery

Filter:
  • Flow cytometry (FC) experiment of Daudi cells using CoraLite® Plus 488-conjugated IL-4R Polyclonal ant (CL488-28331)

    FC experiment of Daudi using CL488-28331

    1x10^6 Daudi cells were intracellularly stained with 0.8 ug Coralite® Plus 488 IL-4r Polyclonal Antibody (CL488-28331)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive FC detected inDaudi cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28331 targets IL-4R in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-4R fusion protein Ag28766 相同性解析による交差性が予測される生物種
Full Name interleukin 4 receptor
Calculated molecular weight 90 kDa
Observed molecular weight 120-140 kDa
GenBank accession numberNM_000418
Gene symbol IL4R
Gene ID (NCBI) 3566
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 IL-4R antibody CL488-28331Download protocol
Standard Protocols
Click here to view our Standard Protocols