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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of Daudi cells using IL-4R/CD124 Recombinant antibody (84414-6-RR)

    IF Staining of Daudi using 84414-6-RR

    Immunofluorescent analysis of (4% PFA) fixed Daudi cells using IL-4R/CD124 antibody (84414-6-RR, Clone: 241824A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84414-6-RR

    Affinity and Kinetic Characterization of 84414-6-RR

    Biolayer interferometry (BLl) kinetic assays of 84414-6-RR against Human IL-4R/CD124 were performed. The affinity constant is below 1 pM.

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IL-4RA/CD124 Recombinant antibody

IL-4RA/CD124 Recombinant Antibody for IF/ICC, ELISA
Cat No. 84414-6-RR
Clone No.241824A11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

IL4R, IL 4R subunit alpha, IL 4R alpha, IL 4 receptor subunit alpha, IL 4 binding protein

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive IF/ICC detected inDaudi cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84414-6-RR targets IL-4RA/CD124 in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 4 receptor
Calculated molecular weight90 kDa
GenBank accession numberNM_000418.4
Gene Symbol IL-4R
Gene ID (NCBI) 3566
RRIDAB_3671943
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP24394-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IF protocol for IL-4RA/CD124 antibody 84414-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols