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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human IL-5 Rabbit Recombinant Antibody

IL-5 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240915D12

Cat no : 98178-1-RR

Synonyms

IL5, Interleukin-5, Interleukin 5, IL 5, B-cell differentiation factor I

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human PBMCs using IL-5 Recombinant antibody (98178-1-RR)

    FC experiment of human PBMCs using 98178-1-RR

    1x10^6 untreated or PMA, Ionomycin and Brefeldin A treated human PBMCs were intracellularly stained with 0.25 ug Anti-Human IL-5 Rabbit Recombinant Antibody (98178-1-RR, Clone: 240915D12) and PE-conjugated Goat Anti-Rabbit IgG. Cells were then stained with CoraLite® Plus 647 Anti-Human CD4. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98178-1-RR

    Affinity and Kinetic Characterization of 98178-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98178-1-RR against Human IL-5 were performed. The affinity constant is 0.689 nM.

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Tested Applications

Positive FC (Intra) detected inPMA, Ionomycin and Brefeldin A treated human PBMCs

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98178-1-RR targets IL-5 in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 5 (colony-stimulating factor, eosinophil)
Calculated molecular weight 134 aa, 15 kDa
GenBank accession numberBC066282
Gene symbol IL5
Gene ID (NCBI) 3567
RRIDAB_3672321
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

IL-5 is a 4-α-helix, pleiotropic cytokine that regulates the activities of B cells, eosinophils, and basophils. It is produced mostly by CD4+ T cells, eosinophils, and basophils, and signals through its specific receptor (IL-5R) on target cells to activate downstream JAK/STAT, Btk, and Ras/ERk pathways. Due to its pleiotropic effects, IL-5 plays a role in both innate and adaptive immune responses. It is a strong promoter of eosinophil differentiation, migration, maturation, and survival. Additionally, IL-5 is also capable of stimulating activated B cells to differentiate into antibody-secreting cells. Elevated IL-5 levels are strongly correlated with eosinophilia, which is a hallmark feature of allergy, asthma, and hypersensitivity. (PMID: 21986312, 22174445, 19819937, 16895557)

Protocols

Product Specific Protocols
FC protocol for IL-5 antibody 98178-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols