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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-6 Polyclonal antibody (30953-1-AP)

    WB analysis using 30953-1-AP

    LPS and Brefeldin A treated NR8383 cells were subjected to SDS PAGE followed by western blot with 30953-1-AP (IL-6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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IL-6 Polyclonal antibody

IL-6 Polyclonal Antibody for WB, ELISA
Cat No. 30953-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

rat

Applications

WB, ELISA

Synonyms

Ifnb2, Il6, IL-6, ILg6, interleukin 6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inLPS and Brefeldin A treated NR8383 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30953-1-AP targets IL-6 in WB, ELISA applications and shows reactivity with rat samples.

Tested Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 6
Calculated molecular weight24 kDa
Observed molecular weight24 kDa
GenBank accession numberNM_012589.2
Gene Symbol Il6
Gene ID (NCBI) 24498
RRIDAB_3669793
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20607
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Protocols

Product Specific Protocols
WB protocol for IL-6 antibody 30953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols