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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-6 Recombinant antibody, PBS Only

IL-6 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240670F10

Cat no : 83747-5-PBS

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using IL-6 Recombinant antibody (83747-5-RR)

    WB analysis of HUVEC using 83747-5-RR (same clone as 83747-5-PBS)

    LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 83747-5-RR (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83747-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using IL-6 Recombinant antibody (83747-5-RR)

    IF Staining of THP-1 using 83747-5-RR (same clone as 83747-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL-6 antibody (83747-5-RR, Clone: 240670F10 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83747-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83747-5-RR

    Affinity and Kinetic Characterization of 83747-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83747-5-RR against Human IL-6 were performed. The affinity constant is 1.60 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83747-5-PBS is IL-6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 6 (interferon, beta 2)
Calculated molecular weight 212 aa, 24 kDa
GenBank accession numberBC015511
Gene symbol IL6
Gene ID (NCBI) 3569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.