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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-6 Recombinant antibody, PBS Only

IL-6 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240670F10

Cat no : 83747-5-PBS

Synonyms

IL6, Interferon beta-2, IL 6, IFN-beta-2, B-cell stimulatory factor 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HUVEC cells using IL-6 Recombinant antibody (83747-5-RR)

    WB analysis of HUVEC using 83747-5-RR (same clone as 83747-5-PBS)

    LPS and Brefeldin A treated HUVEC cells were subjected to SDS PAGE followed by western blot with 83747-5-RR (IL-6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83747-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of THP-1 cells using IL-6 Recombinant antibody (83747-5-RR)

    IF Staining of THP-1 using 83747-5-RR (same clone as 83747-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using IL-6 antibody (83747-5-RR, Clone: 240670F10 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83747-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83747-5-RR

    Affinity and Kinetic Characterization of 83747-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83747-5-RR against Human IL-6 were performed. The affinity constant is 1.60 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83747-5-PBS is IL-6 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 6 (interferon, beta 2)
Calculated molecular weight 212 aa, 24 kDa
Observed molecular weight24 kDa
GenBank accession numberBC015511
Gene symbol IL6
Gene ID (NCBI) 3569
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.