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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Mouse IL-6 Rabbit Recombinant Antibody

IL-6 Recombinant Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC (Intra)

Conjugate

Unconjugated

CloneNo.

240701E8

Cat no : 98089-1-RR

Synonyms

Il6, interleukin-6, interleukin 6, Il 6, 240701E8

Validation Data Gallery

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  • Flow cytometry (FC) experiment of RAW 264.7 cells using Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR)

    FC experiment of RAW 264.7 using 98089-1-RR

    1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L),or 1x10^6 untreated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of RAW 264.7 cells using Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR)

    FC experiment of RAW 264.7 using 98089-1-RR

    1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) (red), or Isotype Control (blue), or 1x10^6 LPS treated RAW 264.7 cells were intracellularly stained with 0.25 ug Anti-Mouse IL-6 Rabbit Recombinant Antibody (98089-1-RR, Clone:240701E8) (green)and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 98089-1-RR

    Affinity and Kinetic Characterization of 98089-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98089-1-RR against Mouse IL-6 were performed. The affinity constant is 0.795 nM.

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Tested Applications

Positive FC (Intra) detected inLPS treated RAW 264.7 cells

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 98089-1-RR is IL-6 Fusion Protein expressed in E. coli.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 6
Calculated molecular weight 24 kDa
GenBank accession numberNM_031168
Gene symbol Il6
Gene ID (NCBI) 16193
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

Background Information

Interleukin-6 (IL-6) is an interleukin that acts as both a pro-inflammatory and anti-inflammatory cytokine. IL-6 protein is secreted by a variety of cell types including T cells and macrophages as phosphorylated and variably glycosylated molecule. IL-6 plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. IL-6 is also considered a myokine, a cytokine produced from muscle, and is elevated in response to muscle contraction. IL-6 has been shown to interact with interleukin-6 receptor and glycoprotein 130. Additionally, IL-6 is involved in hematopoiesis, bone metabolism, and cancer progression, and has been defined an essential role in directing transition from innate to acquired immunity.

Protocols

Product Specific Protocols
FC protocol for IL-6 antibody 98089-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols