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Validation Data Gallery

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  • Western Blot (WB) analysis of RAW 264.7 cells using CD126/IL-6R alpha Recombinant antibody (83688-4-RR)

    WB analysis of RAW 264.7 using 83688-4-RR

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 83688-4-RR (CD126/IL-6R alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 83688-4-RR

    Affinity and Kinetic Characterization of 83688-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83688-4-RR against Human CD126/IL-6R alpha were performed. The affinity constant is 0.80 nM.

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CD126/IL-6R alpha Recombinant antibody

CD126/IL-6R alpha Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83688-4-RR
Clone No.240734D1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, ELISA

Synonyms

CD126, IL-6R alpha, 240734D1, IL 6R alpha, IL 6R 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inRAW 264.7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83688-4-RR targets CD126/IL-6R alpha in WB, ELISA applications and shows reactivity with mouse samples.

Tested Reactivity mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name interleukin 6 receptor
Calculated molecular weight 468 aa, 52 kDa
Observed molecular weight65 kDA
GenBank accession numberBC132684
Gene Symbol IL-6R alpha
Gene ID (NCBI) 3570
RRIDAB_3671290
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP08887
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R, the complex of IL-6 and IL-6R associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while IL-6R is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892).

Protocols

Product Specific Protocols
WB protocol for CD126/IL-6R alpha antibody 83688-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols