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Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD126/IL-6R alpha Monoclonal antibody, PBS Only

CD126/IL-6R alpha Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A5F4

Cat no : 66855-1-PBS

Synonyms

CD126, IL-6R alpha, 2A5F4, IL 6R alpha, IL 6R 1

Validation Data Gallery

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  • Western Blot (WB) analysis of human spleen tissue using CD126/IL-6R alpha Monoclonal antibody (66855-1-Ig)

    WB analysis of human spleen using 66855-1-Ig (same clone as 66855-1-PBS)

    human spleen tissue were subjected to SDS PAGE followed by western blot with 66855-1-Ig (IL6R alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human peripheral blood leukocyte using CD126/IL-6R alpha Monoclonal antibody (66855-1-Ig)

    WB analysis of human peripheral blood leukocyte using 66855-1-Ig (same clone as 66855-1-PBS)

    Human peripheral blood leukocyte were subjected to SDS PAGE followed by western blot with 66855-1-Ig (IL6R alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD126/IL-6R alpha Monoclonal antibody (66855-1-Ig)

    IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using CD126/IL-6R alpha Monoclonal antibody (66855-1-Ig)

    IHC staining of human liver cancer using 66855-1-Ig (same clone as 66855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD126/IL-6R alpha Monoclonal antibody (66855-1-Ig)

    IHC staining of human tonsillitis using 66855-1-Ig (same clone as 66855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using CD126/IL-6R alpha Monoclonal antibody (66855-1-Ig)

    IHC staining of human tonsillitis using 66855-1-Ig (same clone as 66855-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66855-1-Ig (IL6R alpha antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66855-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66855-1-PBS targets CD126/IL-6R alpha in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD126/IL-6R alpha fusion protein Ag18263 相同性解析による交差性が予測される生物種
Full Name interleukin 6 receptor
Calculated molecular weight 468 aa, 52 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC132684
Gene symbol IL-6R alpha
Gene ID (NCBI) 3570
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin-6 (IL-6) is a pleiotropic cytokine produced by a variety of cells during infection, trauma, and immunological challenge (PMID: 8274730). IL-6 acts via a receptor complex consisting of two distinct membrane-bound glycoproteins, an 80-kDa IL-6-binding subunit (IL-6R alpha, CD126, gp80) and a 130-kDa signal-transducing element (gp130, CD130). After binding of IL-6 to membrane-bound IL-6R alpha, the complex of IL-6 and IL-6R alpha associates with gp130, thus activating the receptor (PMID: 9716487). Expression of gp130 is found in almost all organs while expression of IL-6R alpha is predominantly confined to hepatocytes and leukocyte subpopulations (monocytes, neutrophils, T cells, and B cells) (PMID: 11149892). Soluble form of IL-6R alpha has been found in human serum and urine (PMID: 2529343; 1545125).