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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-9 Monoclonal antibody, PBS Only

IL-9 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1F3A11

Cat no : 66144-1-PBS

Synonyms

IL9, Interleukin-9, interleukin9, interleukin 9, IL 9

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-9 Monoclonal antibody (66144-1-Ig)

    WB analysis of Recombinant protein using 66144-1-Ig (same clone as 66144-1-PBS)

    Recombinant protein were subjected to SDS PAGE followed by western blot with 66144-1-Ig (IL9 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-9 Monoclonal antibody (66144-1-Ig)

    IHC staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66144-1-Ig (IL-9 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-9 Monoclonal antibody (66144-1-Ig)

    IHC staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66144-1-Ig (IL-9 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using IL-9 Monoclonal antibody (66144-1-Ig)

    IF Staining of human tonsillitis using 66144-1-Ig (same clone as 66144-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using IL-9 antibody (66144-1-Ig, Clone: 1F3A11 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66144-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66144-1-PBS targets IL-9 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen IL-9 fusion protein Ag21435 相同性解析による交差性が予測される生物種
Full Name interleukin 9
Calculated molecular weight 144 aa, 16 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC066284
Gene symbol IL9
Gene ID (NCBI) 3578
RRIDAB_2881541
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Interleukin 9 (IL-9) is a γc-family cytokine that is highly produced by T-helper 9 (Th9) cells. IL-9 could promote the survival and activation of various cellular targets, including mast cells, B cells, T cells, and structural cells. Its expression is considered a hallmark of Th2-lineage cells. Primarily studied in Th2-type immunity, IL-9 was shown to be involved in asthma, allergy, and host defense against helminth infections. IL-9 also stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes.