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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-10RA Polyclonal antibody (13356-1-AP)

    WB analysis using 13356-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL-10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using IL-10RA Polyclonal antibody (13356-1-AP)

    WB analysis of human liver using 13356-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using IL-10RA Polyclonal antibody (13356-1-AP)

    WB analysis of human heart using 13356-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using IL-10RA Polyclonal antibody (13356-1-AP)

    WB analysis of human placenta using 13356-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 13356-1-AP (IL10RA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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IL-10RA Polyclonal antibody

IL-10RA Polyclonal Antibody for WB, ELISA
Cat No. 13356-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

CD210, CDw210a, HIL 10R, IL 10 receptor subunit alpha, IL 10R subunit 1, IL 10R subunit alpha, IL 10R1, IL 10RA, IL-10 R alpha, IL10R, IL10RA, IL-10RA, interleukin 10 receptor, alpha

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, human heart tissue, human placenta tissue, human liver tissue, K-562 cells, mouse brain tissue, rat brain tissue, rat liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13356-1-AP targets IL-10RA in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-10RA fusion protein Ag4156 相同性解析による交差性が予測される生物種
Full Name interleukin 10 receptor, alpha
Calculated molecular weight 578 aa, 63 kDa
Observed molecular weight 70-72 kDa, 90-95 kDa
GenBank accession numberBC028082
Gene Symbol IL-10RA
Gene ID (NCBI) 3587
RRIDAB_10643390
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13651
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-10 (IL-10) is an anti-inflammatory cytokine that plays a critical role in maintaining immune system balance. IL-10 signals through a receptor complex consisting of two molecules of the IL-10R alpha-chain (IL-10RA) and two molecules of the accessory IL-10R beta-chain (IL-10RB) (PMID: 22236434). IL-10RA and IL-10RB are members of the type II cytokine receptor family. IL-10RA, also known as IL-10R1 or CD210, is expressed on most hematopoietic cells at a basal level but is upregulated by various cells upon activation (PMID: 24507158). IL-10RA serves as the ligand-binding subunit of the receptor complex. Upon binding of IL-10 to the IL-10R complex, the kinases JAK1 and TYK2 are activated and catalyze the phosphorylation of IL-10RA at specific intracellular tyrosine residues, leading to the recruitment and subsequent phosphorylation of STAT3 (PMID: 11244051; 10433356).

Protocols

Product Specific Protocols
WB protocol for IL-10RA antibody 13356-1-APDownload protocol
FC protocol for IL-10RA antibody 13356-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Immunol

The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy

Authors - Yanli Ma
View Article