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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-12A Monoclonal antibody

IL-12A Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

6A10A8

Cat no : 60324-1-Ig

Synonyms

CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL12A, IL-12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35

Validation Data Gallery

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  • Western Blot (WB) analysis of Recombinant protein using IL-12A Monoclonal antibody (60324-1-Ig)

    WB analysis of Recombinant protein using 60324-1-Ig

    Recombinant protein were subjected to SDS PAGE followed by western blot with 60324-1-Ig (IL12A Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using IL-12A Monoclonal antibody (60324-1-Ig)

    IHC staining of human kidney using 60324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using IL-12A Monoclonal antibody (60324-1-Ig)

    IHC staining of human kidney using 60324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-12A Monoclonal antibody (60324-1-Ig)

    IHC staining of human tonsillitis using 60324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using IL-12A Monoclonal antibody (60324-1-Ig)

    IHC staining of human tonsillitis using 60324-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60324-1-Ig (IL12A Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inRecombinant protein
Positive IHC detected inhuman kidney tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60324-1-Ig targets IL-12A in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen IL-12A fusion protein Ag17625 相同性解析による交差性が予測される生物種
Full Name interleukin 12A (natural killer cell stimulatory factor 1, cytotoxic lymphocyte maturation factor 1, p35)
Calculated molecular weight 25 kDa
GenBank accession numberBC104982
Gene symbol IL-12A
Gene ID (NCBI) 3592
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL12A is a subunit of a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. The cytokine is a disulfide-linked heterodimer composed of the 35-kD subunit encoded by this gene, and a 40-kD subunit that is a member of the cytokine receptor family. This cytokine is required for the T-cell-independent induction of IFN-gamma, and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4. Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity.

Protocols

Product Specific Protocols
WB protocol for IL-12A antibody 60324-1-IgDownload protocol
IHC protocol for IL-12A antibody 60324-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols