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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-15RA Monoclonal antibody

IL-15RA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

5E3E1

Cat no : 60138-1-Ig

Synonyms

IL 15 receptor subunit alpha, IL 15R alpha, IL 15RA, IL15RA, IL-15RA, interleukin 15 receptor, alpha, sIL 15 receptor subunit alpha, sIL 15R alpha, sIL 15RA

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using IL-15RA Monoclonal antibody (60138-1-Ig)

    WB analysis of Raji using 60138-1-Ig

    Raji cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Daudi cells using IL-15RA Monoclonal antibody (60138-1-Ig)

    WB analysis of Daudi using 60138-1-Ig

    Daudi cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using IL-15RA Monoclonal antibody (60138-1-Ig)

    WB analysis of HeLa using 60138-1-Ig

    HeLa cells were subjected to SDS PAGE followed by western blot with 60138-1-Ig (IL15RA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inRaji cells, Daudi cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60138-1-Ig targets IL-15RA in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen IL-15RA fusion protein Ag10200 相同性解析による交差性が予測される生物種
Full Name interleukin 15 receptor, alpha
Calculated molecular weight 231 aa, 24 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC107777
Gene symbol IL-15RA
Gene ID (NCBI) 3601
RRIDAB_2881352
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-15 (IL-15) is a pleiotropic cytokine that plays an important role in both innate and adaptive immunity. IL-15 is mainly produced by activated monocytes, macrophages and dendritic cells, and is structurally similar to IL-2 (PMID: 8178155; 12401478). These two cytokines share the same IL-2/15Rβ and common γ-chain receptor subunits. In addition, IL-2 and IL-15 have their own private α-chain receptor subunit, IL-2Rα and IL-15Rα, respectively (PMID: 7641685). The IL-15Rα chain is expressed by many cell types including monocytes, DCs, NK, T cells and fibroblasts. Several isoforms of IL-15Rα exist and are generated either by alternative splicing or by proteolytic cleavage (PMID: 10480910; 15265897). The calculated molecular weight of full-length IL-15Rα is 28 kDa, IL-15Rα can be glycosylated, and larger apparent molecular weights of 55-65 kDa have been reported by some researches (PMID: 10480910; 15976182; 15671076; 17912462).

Protocols

Product Specific Protocols
WB protocol for IL-15RA antibody 60138-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols