Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, NIH/3T3 cells, HaCaT cell, HaCaT cells, DU 145 cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HeLa cells |
| Positive FC (Intra) detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 296 publications below |
| IHC | See 49 publications below |
| IF | See 30 publications below |
| IP | See 1 publications below |
| FC | See 1 publications below |
Product Information
10663-1-AP targets IL-18 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IL-18 fusion protein Ag1063 相同性解析による交差性が予測される生物種 |
| Full Name | IL-18 |
| Calculated molecular weight | 193 aa, 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC007461 |
| Gene Symbol | IL-18 |
| Gene ID (NCBI) | 3606 |
| RRID | AB_2123636 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14116 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
IL18, is a proinflammatory cytokine involved in the development of Th1 cells and in immune response. It can stimulate the NK cells and certain T cells to release IFN gamma which plays an important role in activating the macrophages or other cells. IL18 has been demonstrated to have the potential to enhance Fas ligand-mediated cytotoxicity, which is increased in PE and regulates placental apoptosis. IL18 is synthesized as a 22 kDa precursor and then cleaved into a biologically activate 18 kDa form. This antibody 10663-1-AP is raised against the full length IL18 precursor, and it recognizes both the pre-IL18 and mature IL18 theoretically.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-18 antibody 10663-1-AP | Download protocol |
| IF protocol for IL-18 antibody 10663-1-AP | Download protocol |
| IP protocol for IL-18 antibody 10663-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv FUNDC1 interacts with FBXL2 to govern mitochondrial integrity and cardiac function through an IP3R3-dependent manner in obesity. | ||
Nat Commun IL-22 initiates an IL-18-dependent epithelial response circuit to enforce intestinal host defence. | ||
J Pineal Res Melatonin inhibits atherosclerosis progression via Galectin-3 downregulation to enhance autophagy and inhibit inflammation | ||
Aging Dis Sterol-resistant SCAP Overexpression in Vascular Smooth Muscle Cells Accelerates Atherosclerosis by Increasing Local Vascular Inflammation through Activation of the NLRP3 Inflammasome in Mice. | ||
Proc Natl Acad Sci U S A Telomere dysfunction instigates inflammation in inflammatory bowel disease. |