IL-1 alpha Polyclonal antibody

Name: IL-1 alpha Polyclonal antibody
Brand: Proteintech
SKU: 16765-1-AP

IL-1 alpha Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 16765-1-AP

Synonyms

IL1A, IL 1 alpha, Hematopoietin-1, IL-1,IL1, IL1 ALPHA

Validation Data Gallery

Filter:

AllWBIHCIF/ICC

WB analysis using 16765-1-AP
Untreated THP-1 cells, THP-1 cells (treated with 50 nM PMA and 5 ug/ml LPS for 24 hours, with addition of 300ng/ml Brefeldin A for the last 3 hours.) were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL-1 alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 16765-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL 1 alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse lung using 16765-1-AP
mouse lung tissue were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL 1 alpha antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 16765-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 16765-1-AP (IL 1 alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IHC staining of human appendicitis using 16765-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16765-1-AP (IL-1 alpha antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human appendicitis using 16765-1-AP
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 16765-1-AP (IL-1 alpha antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of RAW 264.7 using 16765-1-AP
Immunofluorescent analysis of (-20℃ Ethanol) fixed RAW 264.7 cells using 16765-1-AP (IL 1 alpha antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Cat No. 16765-1-AP

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	PMA, LPS and Brefeldin A treated THP-1 cells, human placenta tissue, mouse liver tissue, mouse lung tissue
Positive IHC detected in	human appendicitis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	RAW 264.7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 22 publications below
IHC	See 13 publications below
IF	See 10 publications below
ELISA	See 1 publications below

Product Information

16765-1-AP targets IL-1 alpha in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	IL-1 alpha fusion protein Ag10339 相同性解析による交差性が予測される生物種
Full Name	interleukin 1, alpha
Calculated molecular weight	271 aa, 31 kDa
Observed molecular weight	30 kDa
GenBank accession number	BC013142
Gene symbol	IL-1 alpha
Gene ID (NCBI)	3552
RRID	AB_10641044
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Interleukin-1α (IL-1 alpha) is a protein that plays a crucial role in the regulation of a number of key cellular processes. IL-1 Alpha is a cytokine and a potent mediator of body's response to inflammation, microbial invasion, tissue injury and immunological response. In addition, recent studies suggest that IL-1 Alpha also has a role in wound healing, rheumatoid arthritis, Alzheimer's disease and tumor growth. IL-1 Alpha functions as an alarmin during the process of sterile inflammation. It serves as a signal from dying cells to potentiate the inflammatory response, increasing the expression of other secreted factors like IL-6 and IL-8. IL-1 Alpha was found to affect the cell cycle of osteosarcoma cell to reduce cell growth. IL-1 Alpha is initially translated as approximately 30-kD polypeptides that are processed to approximately 17.5-kD polypeptides prior to, or during, release from macrophages.

Protocols

Product Specific Protocols
WB protocol for IL-1 alpha antibody 16765-1-AP	Download protocol
IHC protocol for IL-1 alpha antibody 16765-1-AP	Download protocol
IF protocol for IL-1 alpha antibody 16765-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	IHC	Nat Commun A stress-induced cilium-to-PML-NB route drives senescence initiation Authors - Xiaoyu Ma View Article
human	IF	Nat Commun Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment Authors - Meilin Xue View Article
human	WB	Gut Microbes Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway Authors - Jian-Wei Zhang View Article
		Cancer Lett Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer Authors - Prabhakar Pitta Venkata View Article
rat	WB	Bioact Mater Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration. Authors - Liwen Luo View Article
human	IF	Cell Death Discov Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer. Authors - Lulin Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1 alpha Polyclonal antibody

IL-1 alpha Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Validation Data Gallery

WB analysis using 16765-1-AP

WB analysis of mouse liver using 16765-1-AP

WB analysis of mouse lung using 16765-1-AP

WB analysis of human placenta using 16765-1-AP

IHC staining of human appendicitis using 16765-1-AP

IHC staining of human appendicitis using 16765-1-AP

IF Staining of RAW 264.7 using 16765-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

A stress-induced cilium-to-PML-NB route drives senescence initiation

Schwann cells regulate tumor cells and cancer-associated fibroblasts in the pancreatic ductal adenocarcinoma microenvironment

Fusobacterium nucleatum promotes esophageal squamous cell carcinoma progression and chemoresistance by enhancing the secretion of chemotherapy-induced senescence-associated secretory phenotype via activation of DNA damage response pathway

Pharmacological inhibition of KDM1A/LSD1 enhances estrogen receptor beta-mediated tumor suppression in ovarian cancer

Injectable cartilage matrix hydrogel loaded with cartilage endplate stem cells engineered to release exosomes for non-invasive treatment of intervertebral disc degeneration.

Senescence as a dictator of patient outcomes and therapeutic efficacies in human gastric cancer.