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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1 beta Polyclonal antibody

IL-1 beta Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 30234-1-AP

Synonyms

Catabolin, IL 1, IL 1 beta, IL1 BETA, IL1B, IL1beta, IL1F2, Interleukin 1 beta, interleukin 1, beta

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-1 beta Polyclonal antibody (30234-1-AP)

    WB analysis using 30234-1-AP

    Untreated, LPS and Protein transport inhibitor treated THP-1 cells were subjected to SDS PAGE followed by western blot with 30234-1-AP (IL1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-87 MG cells using IL-1 beta Polyclonal antibody (30234-1-AP)

    WB analysis of U-87 MG using 30234-1-AP

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 30234-1-AP (IL1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inTHP-1 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30234-1-AP targets IL-1 beta in WB, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-1 beta fusion protein Ag30574 相同性解析による交差性が予測される生物種
Full Name interleukin 1, beta
Calculated molecular weight 31 kDa
Observed molecular weight31 kDa
GenBank accession numberNM_000576
Gene symbol IL1B
Gene ID (NCBI) 3553
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response. It is identical to endogenous pyrogen. The multiple biologic activities that define IL1 are properties of a 15- to 18-kD protein that is derived from a 30- to 35-kD precursor. Interleukin 1β (IL-1B) is a member of the interleukin 1 cytokine family. It is a pro-inflammatory cytokine against infection, playing an important role in the pathogenesis of cancers. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets

Protocols

Product Specific Protocols
WB protocol for IL-1 beta antibody 30234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols