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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1 beta Recombinant antibody, PBS Only (Capture/Detector)

IL-1 beta Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

2G9

Cat no : 82696-8-PBS

Synonyms

IL1- beta, IL1 beta, IL 1 beta, IL 1, Catabolin

Validation Data Gallery

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  • Sandwich ELISA standard curve of MP00331-2

    Sandwich ELISA standard curve of MP00331-2

    Sandwich ELISA standard curve of MP00331-2, Human IL-1 beta Recombinant Matched Antibody Pair - PBS only. 82696-15-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0286. 82696-8-PBS was HRP conjugated as the detection antibody. Range: 7.8-500 pg/mL.

  • Sandwich ELISA standard curve of MP80001-6

    Sandwich ELISA standard curve of MP80001-6

    Sandwich ELISA standard curve of MP80001-6, IL-1 beta Hybrid Matched Antibody Pair, PBS Only. Capture antibody: 82696-8-RR. Detection antibody: HRP-conjugated 66737-1-PBS. Standard:HZ-1164. Range: 0.098-12.5 ng/mL.

  • Sample test of MP00331-2

    Sample test of MP00331-2

    Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA or 10 μg/mL PHA and 10 ng/mL IL-2 for 1 day. The mean IL-1 beta concentration was not determined in unstimulated PBMC supernatant, 5,709.3 pg/mL in PHA stimulated PBMC supernatant and 1,110.8 pg/mL in PHA and IL-2 stimulated PBMC supernatant.

  • Sample test of MP00331-2

    Sample test of MP00331-2

    Serum of four individual healthy human donors was measured. The IL-1 beta concentration of detected samples was determined to be 93.4 pg/mL with a range of 20.3 - 131.0 pg/mL.

  • Cytometric bead array standard curve of MP00331-2

    Cytometric bead array standard curve of MP00331-2

    Cytometric bead array standard curve of MP00331-2, IL-1 beta Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82696-15-PBS. Detection antibody: 82696-8-PBS. Standard: Eg0286. Range: 0.313-40 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82696-8-PBS targets IL-1 beta as part of a matched antibody pair:

MP00331-2: 82696-15-PBS capture and 82696-8-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

MP80001-6: 82696-8-PBS capture and 66737-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name interleukin 1, beta
Calculated molecular weight 269 aa, 31 kDa
GenBank accession numberBC008678
Gene symbol IL1B
Gene ID (NCBI) 3553
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.