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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1RAP Polyclonal antibody

IL-1RAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30966-1-AP

Synonyms

IL1RAP, IL-1 receptor accessory protein, IL 1RAcP, IL 1R3, IL 1R 3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-1RAP Polyclonal antibody (30966-1-AP)

    WB analysis using 30966-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30966-1-AP (IL-1RAP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IL-1RAP Polyclonal antibody (30966-1-AP)

    IHC staining of human ovary cancer using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using IL-1RAP Polyclonal antibody (30966-1-AP)

    IHC staining of human ovary cancer using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using IL-1RAP Polyclonal antibody (30966-1-AP)

    IHC staining of human placenta using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using IL-1RAP Polyclonal antibody (30966-1-AP)

    IHC staining of human placenta using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using IL-1RAP Polyclonal antibody (30966-1-AP)

    IHC staining of mouse liver using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human intrahepatic cholangiocarcinoma tissue using IL-1RAP Polyclonal antibody (30966-1-AP)

    IHC staining of human intrahepatic cholangiocarcinoma using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using IL-1RAP Polyclonal antibody (30966-1-AP)

    IHC staining of mouse liver using 30966-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 30966-1-AP (IL-1RAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHeLa cells, human placenta tissue, mouse liver tissue
Positive IHC detected inhuman placenta tissue, human intrahepatic cholangiocarcinoma tissue, human ovary cancer tissue, mouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30966-1-AP targets IL-1RAP in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-1RAP fusion protein Ag34121 相同性解析による交差性が予測される生物種
Full Name interleukin 1 receptor accessory protein
Calculated molecular weight65 kDa
Observed molecular weight70 kDa
GenBank accession numberNM_001167928.1
Gene symbol IL1RAP
Gene ID (NCBI) 3556
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IL-1RAP (Interleukin-1 receptor accessory protein), also known as IL-1RAcP, IL-1R-3, or IL-1R3, is a member of the interleukin-1 receptor family. IL-1RAP is composed of an extracellular region containing three Ig-like C2 type domains, a transmembrane region, and a cytoplasmic region with a TIR (Toll-IL-1-receptor) domain. IL-1RAP functions as a co-receptor for IL-1, IL-33, IL-36. It plays an essential role in the signaling of the IL-1 family cytokines such as IL-1, IL-33, and IL-36, as well as tyrosine kinases FLT3 and c-Kit. IL-1RAP can also exist in a soluble form.

Protocols

Product Specific Protocols
WB protocol for IL-1RAP antibody 30966-1-APDownload protocol
IHC protocol for IL-1RAP antibody 30966-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols