Validation Data Gallery
Filter:
Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 3 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
Product Information
21609-1-AP targets IL-1-RAPL-1 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | IL-1-RAPL-1 fusion protein Ag16252 相同性解析による交差性が予測される生物種 |
| Full Name | interleukin 1 receptor accessory protein-like 1 |
| Calculated molecular weight | 696 aa, 80 kDa |
| Observed molecular weight | 80-100 kDa |
| GenBank accession number | BC126345 |
| Gene Symbol | IL-1-RAPL-1 |
| Gene ID (NCBI) | 11141 |
| RRID | AB_10888632 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NZN1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IL-1-RAPL-1 antibody 21609-1-AP | Download protocol |
| IHC protocol for IL-1-RAPL-1 antibody 21609-1-AP | Download protocol |
| IF protocol for IL-1-RAPL-1 antibody 21609-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay.
| ||
Allergy Skewed inflammation is associated with aberrant interleukin-37 signaling pathway in atopic dermatitis. | ||
EMBO J Splice-dependent trans-synaptic PTPδ-IL1RAPL1 interaction regulates synapse formation and non-REM sleep. | ||
Cell Death Dis IL-37 isoform D downregulates pro-inflammatory cytokines expression in a Smad3-dependent manner.
| ||
J Neurosci The X-linked intellectual disability protein IL1RAPL1 regulates dendrite complexity.
| ||
Cell Rep IL-38 Ameliorates Skin Inflammation and Limits IL-17 Production from γδ T Cells. |