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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

IL-1RA Polyclonal antibody

IL-1RA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 10844-1-AP

Synonyms

IL 1ra, IL1RN, IL-1RN, IL1RA, IL 1RN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using IL-1RA Polyclonal antibody (10844-1-AP)

    WB analysis using 10844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using IL-1RA Polyclonal antibody (10844-1-AP)

    WB analysis using 10844-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10844-1-AP (IL-1RA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using IL-1RA Polyclonal antibody (10844-1-AP)

    IHC staining of human oesophagus using 10844-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus tissue using IL-1RA Polyclonal antibody (10844-1-AP)

    IHC staining of human oesophagus using 10844-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using IL-1RA Polyclonal antibody (10844-1-AP)

    IHC staining of human colon using 10844-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 10844-1-AP (IL-1RA antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using IL-1RA Polyclonal antibody (10844-1-AP)

    IHC staining of human colon cancer using 10844-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 10844-1-AP (IL1RA antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using IL-1RA Polyclonal antibody (10844-1-AP)

    IHC staining of human stomach using 10844-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using IL-1RA Polyclonal antibody (10844-1-AP)

    IHC staining of human colon using 10844-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using IL-1RA Polyclonal antibody (10844-1-AP)

    IHC staining of human stomach using 10844-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using IL-1RA Polyclonal antibody (10844-1-AP)

    IHC staining of human stomach using 10844-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using IL-1RA Polyclonal antibody (10844-1-AP)

    IHC staining of human colon using 10844-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10844-1-AP (IL1RA antibody at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using IL-1RA Polyclonal antibody (10844-1-AP)

    IF Staining of A431 using 10844-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using IL-1RA antibody (10844-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of A431 cells using IL-1RA Polyclonal antibody (10844-1-AP)

    FC experiment of A431 using 10844-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human IL-1RA (10844-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells, MOLT-4 cells, RAW 264.7 cells
Positive IHC detected inhuman oesophagus tissue, human colon tissue, human colon cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10844-1-AP targets IL-1RA in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-1RA fusion protein Ag1277 相同性解析による交差性が予測される生物種
Full Name interleukin 1 receptor antagonist
Calculated molecular weight 20 kDa
Observed molecular weight18-20 kDa
GenBank accession numberBC009745
Gene symbol IL-1RA
Gene ID (NCBI) 3557
RRIDAB_2125559
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for IL-1RA antibody 10844-1-APDownload protocol
IHC protocol for IL-1RA antibody 10844-1-APDownload protocol
IF protocol for IL-1RA antibody 10844-1-APDownload protocol
FC protocol for IL-1RA antibody 10844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Clin Transl Oncol

Inhibitory effect and mechanism of mesenchymal stem cells on melanoma cells.

Authors - J Zhang
View Article
humanWB

NeuroImmune Pharm Ther

Resveratrol and its analogs suppress HIV replication, oxidative stress, and inflammation in macrophages

Authors - Santosh Kumar
View Article
humanIHC

Breast Cancer (Dove Med Press)

Identification and Validation of Novel Metastasis-Related Immune Gene Signature in Breast Cancer

Authors - Shen Ma
View Article
mouseIF

Front Immunol

The influence of angiopoietin-like protein 3 on macrophages polarization and its effect on the podocyte EMT in diabetic nephropathy

Authors - Yanli Ma
View Article