IL1RN Recombinant antibody, PBS Only (Capture)

Sandwich ELISA standard curve of MP01389-3, Human IL-1RA Recombinant Matched Antibody Pair - PBS only. 84532-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1277. 84532-3-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated or stimulated with 10 μg/mL PHA for 3 days. The mean IL-1RA concentration was determined to be 4,505.9 pg/mL in unstimulated PBMC supernatant, 74,132.2 pg/mL in PHA stimulated PBMC supernatant.

HepG2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human IL-1RA, and measured 534.4 pg/mL.

Serum of six individual healthy human donors was measured. The IL-1RA concentration of detected samples was determined to be 637.3 pg/mL with a range of 49.7-1,417.8 pg/mL.