IL-2 Recombinant antibody, PBS Only

Untreated, PMA, A23187 and Brefeldin A treated Jurkat cells were subjected to SDS PAGE followed by western blot with 83558-5-RR (IL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83558-5-PBS in a different storage buffer formulation.

Biolayer interferometry (BLl) kinetic assays of 83558-5-RR against Human IL-2 were performed. The affinity constant is below 1 pM.